ABSTRACT
Objective:To analyze the diversity of Bacteroides in simple obesity children aged 7-14 and the difference from that of normal individuals.And to explore the influence of children's simple obesity on intestinal advantage bacterium of the genus.Methods:We have collected fecal samples of 30 cases with simple obesity aged 7 to 14 years old and 30 healthy children in the Central Hospital of Shan County.All bacterial DNAs were extracted from the feces, and then Bacteroides 16s DNA specific primers was used in PCR amplification, separated by DGGE, the intestinal Bacteroides molecular fingerprints of obese children and healthy children were obtained, and the diversity and similarity were analyzed.At the same time, the weight, height, BMI, blood pressure, blood lipid, leptin, plasma adiponectin in the obesity and normal children were measured, the relationship between the obese children and the advantage of intestinal bacteria genera and its clinical significance were investigated.Results:The BMI[(25.71±3.28)kg/m 2], SBP[(121±12.4)mmHg], TG[(1.15±0.40)mmol/L], TC[(4.20±0.73)mmol/L], LEP(18.94±1.68) in the obesity group were higher than those in the normal group [BMI(17.38±3.54)kg/m 2, SBP(104±7.3)mmHg, TG(0.87±0.31)mmol/L, TC(3.37±0.86)mmol/L, LEP(11.29±2.41)](all P<0.05). The levels of HDL[(1.01±0.28)mmol/L] and ADP(4.10±2.32) in the obesity group were lower than those in the normal group [HDL(1.40±0.26)mmol/L, ADP(5.84±3.42)]( P<0.05). There were no statistically significant differences in age, DBP and LDL levels between the two groups (all P>0.05). The stripe number of Bacteroides DGGE profiles (9.33±1.37) and H index(2.07±0.16) in the obesity group were lower than those in the normal group [(17.33±2.80), (2.67±0.20)]. Cluster analysis results showed that the similarities of obese individuals in the group and compared with normal group were reduced.The S and H index of DGGE profiles in Bacteroides were negatively correlated with BMI and LEP ( P<0.01), and positively correlated with ADP( P<0.05). S, H index of the normal group were negatively correlated with LEP ( P<0.05), and had no correlation with BMI, ADP( P>0.05). S and H index of the obesity group were negatively correlated with BMI and LEP, and had no correlation with ADP ( P>0.05). Conclusion:Human intestinal flora of Bacteroides changes is related to childhood obesity, the diversity and similarity of obese children's intestinal Bacteroides species are decreased.The diversity of obesity children's Bacteroides is associated with BMI, ADP and LEP.
ABSTRACT
Objective To investigate the dynamic changes of serum interleukin (IL)-1β,tumor necrosis factor (TNF)-α,cyclooxygenase (COX)-2 levels in patients with acute gouty arthritis with various severityes and to provide evidence for the course of anti-inflammatory drug used in patients with acute gouty arthritis.Methods Ninety patients with gouty arthritis were randomly selected including sixty acute gouty arthritis patients and thirty gout patients in remission (observation group 3).Sixty acute gouty arthritis patients were divided into severe (observation group 1) and mild (observation group 2) subgroups according to the chief complaints and pain score points (VRS-4 method).All acute gouty arthritis patients were given the same dose and duration of etoricoxib and colchicine (14 days in all).Serum IL-1β,TNF-α,COX-2 levels were detected by enzyme-linked immunosorbent assay (ELISA) after the 1,3,7,10 and 14 days after onset.The 2-tailed independent samples t test was used for the analysis of measurement data.ANOVA were adapted for the comparison between groups.Results The serum levels of IL-1β,TNF-α in the observation group 1,2 were significantly higher than those in the control group in day 1 and 7 (P＜0.01),and almost completely returned to normal at day 10 to 14 day anti-inflammatory therapy.The serum COX-2 levels in observation group 1,2 were significantly higher than those in the control group (P＜0.01) in day 1 to 3,and returned to normal at day 7 to 10 after anti-inflammatory therapy.There were no significant differences between observation group3 and the control group (t=-0.880,-1.201,-0.548; P=0.383,0.235,0.586).② The serum levels of IL-1β,TNF-α were significantly higher in observation group 1 than those in observation group 2 [(24±5) pg/ml and(19±3) pg/ml,(323±84) ng/ml and (234±29) ng/ml; P=0.001,0.002],while there was no significant differences between observation group 2 and the control group (P=0.357).The serum COX-2 levels in observation group 1 were significantly higher than those in observation group 2 at day 7 [(12.9±2.0) pg/ml and (9.1±1.6) pg/ml],while there was no significant difference between observation group 2 and the control group (P=0.941).Conclusion Inflammatory factor can return to normal completely after anti-inflammatory therapy for acute gouty arthritis for 10 to 14 days.This study provides evidence for the course of anti-inflammatory drug used in patients with acute gouty arthritis.
ABSTRACT
Objective To investigate the changes in the expression of Nogo-A protein in the dorsal root ganglion (DRG) and spinal dorsal horn in a rat model of neuropathic pain (NP) .Methods Seventy-two male SD rats weighing 250-300 g were randomly divided into 3 groups ( n = 24 each) : control group (group C) , sham operation group (group S) and NP group. NP was induced by ligation and severance of tibial and common fibular nerves according to the technique described by Isabelle et al. The mechanical withdrawal threshold (MWT) to von Frey filament stimulation was measured at 1, 7, 14 and 21 days after ligation. Six rats in each group were randomly selected at each time point and sacrificed (3 for determination of Nogo-A protein expression by immunofluorescence, 3 for determination of Nogo-A protein expression by Western blot) . The L5 DRG and L4,5 segment of spinal cord on the injured side were removed for determination of Nogo-A protein expression by immunofluorescence and Western blot. Results Compared with the groups C and S, MWT was significantly decreased at 7, 14 and 21 days after ligation, the expression of Nogo-A protein in the DRG was down-regulated at 7 and 14 days after ligation and the expression of Nogo-A protein in the spinal dorsal horn was up-regulated at 14 and 21 days after ligation ( P ＜0.05) .Conclusion The Nogo-A protein in the DRG and spinal dorsal horn may play an important role in peripheral nerve injury-induced NP in rats.